size distribution of plant cell aggregates in batch

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Size distribution of plant cell aggregates in batch ...

1987-5-1  The Chemical Engineering Journal, 35 (1987) B9 - B14 Size Distribution of Plant Cell Aggregates in Batch Culture F. MAVITUNA and J. M. PARK Chemical Engineering Department, UMIST, Sackuille Street, P.O. Box 88, Manchester M60 IQD (U.K.) (Received August 25, 1985; in final form October 17, 1986) B9 ABSTRACT Plant cells in suspension cultures tend to form aggregates.

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Modelling size distribution changes of plant cell ...

2016-7-12  For the modelling of the size distribution change of plant cell aggregates during batch growth, the theory involved the integration of biological growth kinetics, with or without mass transfer resistance, and population balance for particles.

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The Chenrical Engineering Journal, 35 (1987) B9- B14 Size ...

Plant cells in suspension cultures tend to form aggregates. This can affect the physiol-ogy of the cells and hence the production of valuable secondary metabolites. Aggregation is also important from a process engineering point of view. In this work, the change in the size distribution of plant cell aggregates in a batch culture is studied.

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Modelling size distribution changes of plant cell ...

The aim of this work was to develop a theoretical model and its computer simulation for the prediction of plant cell aggregate size distribution change with time in batch suspension cultures. We combined the theory of the particle size distribution with the Monod kinetics to describe the plant cell aggregate growth.

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Simulation of Size Distribution Changes of Plant Cell ...

Simulation of Size Distribution Changes of Plant Cell Aggregates during Batch Growth . By ...

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Characterization of aggregate size in ... - Plant Cell Reports

2010-3-9  Plant cells grow as aggregates in suspension culture, but little is known about the dynamics of aggregation, and no routine methodology exists to measure aggregate size. In this study, we evaluate several different methods to characterize aggregate size in Taxus suspension cultures, in which aggregate diameters range from 50 to 2,000 μm, including filtration and image analysis, and develop a ...

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Development of Plant Cell Culture Processes to Produce ...

2020-11-5  and predict these changes in the aggregate size distribution. This multi-scale engineering approach towards understanding plant cell aggregation serves as an important step in the development of rational strategies aimed at controlling the process variability which has heretofore limited the application of plant cell culture technology.

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Characterization of aggregate size in Taxus suspension ...

2011-7-4  In order to engineer plant cell cultures by manipulating aggregate size, it is necessary to first have a practical and reliable method to measure the aggregate size distribution. Dry weight is the standard measure of biomass in plant cell culture, and the most common method to measure the aggregate size distribution is similar in nature. A crude

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Improved assessment of aggregate size in Taxus plant cell ...

2012-8-28  The valid measurement of plant cell aggregates, however, is not trivial, due to a rather large size distribution and measurement artifacts implied by the measuring method. In this study, laser diffraction was used as a novel method for characterization of Taxus chinensis cells, a major source for the antitumor agent paclitaxel.

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(PDF) Effect of aggregate size in cell cultures of ...

The size of shear-sensitive plant cell aggregates influenced by hydrodynamic forces resulting from mechanical agitation was often recognized as an intangible parameter,

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The Chenrical Engineering Journal, 35 (1987) B9- B14 Size ...

Plant cells in suspension cultures tend to form aggregates. This can affect the physiol-ogy of the cells and hence the production of valuable secondary metabolites. Aggregation is also important from a process engineering point of view. In this work, the change in the size distribution of plant cell aggregates in a batch culture is studied.

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Variation of aggregate size in plant cell suspension batch ...

1993-3-1  Variation of aggregate size in plant cell suspension batch and semi-continuous cultures. Kieran PM, Malone DM, MacLoughlin PF. Food and Bioproducts processing., 01 Mar 1993, 71: 40-46 AGR: IND93042998 . Share this article Share with email Share with twitter Share ...

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Characterization of aggregate size in Taxus suspension ...

2010-3-9  Mavituna F, Park JM. Size distribution of plant cell aggregates in batch culture. Chem Eng J. 1987; 35:B9–B14. [Google Scholar] McDonald KA, Jackman AP, Hurst S. Characterization of plant suspension cultures using the focused beam reflectance technique. Biotechnol Lett. 2001; 23:317–324. [Google Scholar] Naill MC, Roberts SC.

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Cluster size distribution of cell aggregates in culture ...

The growth patterns of established normal and cancer cell lines, cultured in monolayer and collagen gel, have been characterized using the cluster size distribution of cellular aggregates. HN-5 (cancer) cells exhibit, either in gel or in monolayer, power-law distributions at any time in culture, whereas for MDCK (“normal”) and Hep-2 (cancer) cells there is a transition from an exponential ...

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(PDF) Characterization of Aggregate Size in Taxus ...

The size distribution of cell aggregates, and the effect of cell aggregate size on anthocyanin content of Daucus carota cells in suspension cultures, was studied. The profile of biomass ...

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(PDF) On the Theoretical Determination of Optimal Times ...

The effect of hydrodynamic shear stress on aggregate size of plant cell in suspension culture was investigated. Kinetic equations regarding the growth rate of cells within an aggregate were proposed.

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Frequency, size, and localization of bacterial aggregates ...

Bacterial cells were not randomly distributed on the leaf surface but occurred in a wide range of cluster sizes, ranging from single cells to over 10(4) cells per aggregate. The average cluster size increased through time, and aggregates were more numerous and larger when plants were maintained under conditions of high relative humidity levels ...

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Effect of Adjusting for Particle-Size Distribution of ...

2018-4-15  2.2. Approach. To evaluate the effect of the classifications of particle-size distribution of cement, the mix proportion was newly required. Nine mix proportions by the amount of different sizes of cement were classified based on the accumulated remains

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Effect of aggregate size in cell cultures of Tagetes ...

SummaryThe effect of the size of Tagetes patula (marigolds) cell aggregates on growth and thiophene production in MS-medium was studied. A heterogeneous aggregate suspension was aseptically divided into 7 fractions, each with a defined aggregate diameter range, with subsequent inoculation of the fractions into MS growth medium. Growth occurred in all aggregate fractions and thiophene ...

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Porositv and Pore-Size Distribution of Soil Aggregates

2016-5-18  Effect of aggregate size on aggregate porosity 14 Effect of soil type on aggregate porosity. • . 18 Aggregate porosity of virgin and cultivated soils 18 Aggregate porosity of natural and artificial aggregates 21 Comparison of glassbead displacement and thin section methods for determining aggregate porosity 22 Pore-size distribution ...

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Frequency, Size, and Localization of Bacterial Aggregates ...

2020-12-17  Using epifluorescence microscopy and image analysis, we have quantitatively described the frequency, size, and spatial distribution of bacterial aggregates on leaf surfaces of greenhouse-grown bean plants inoculated with the plant-pathogenic bacterium Pseudomonas syringae pv. syringae strain B728a. Bacterial cells were not randomly distributed on the leaf surface but occurred in a wide range ...

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Analysis of aggregate size as a process variable affecting ...

The aggregate size distributions were variable over multiple subculture cycles, and comparison of crude size distributions determined on the basis of dry weight for each aggregate size range indicated that cultures in Fig. 2a contained a significantly higher percentage of smaller aggregates than the cultures in Fig. 2b (Fig. 2c). Total ...

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Aggregates Sizer - Specs : SHIMADZU (Shimadzu Corporation)

2021-5-24  Samples for which the particle size distribution would be changed by dilution can be measured in their original state, or with the minimum required level of dilution, and true images of the measurement object can be obtained. ... If a standard flow cell or batch cell is used to measure a sample at a high concentration, the long light path ...

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Cluster size distribution of cell aggregates in culture

Downloadable (with restrictions)! The growth patterns of established normal and cancer cell lines, cultured in monolayer and collagen gel, have been characterized using the cluster size distribution of cellular aggregates. HN-5 (cancer) cells exhibit, either in gel or in monolayer, power-law distributions at any time in culture, whereas for MDCK (“normal”) and Hep-2 (cancer) cells there is ...

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Determination of Size Distribution of Precipitation ...

These forces affect the true size of the aggregates formed in a unit operation, thereby losing representativity in terms of aggregates particle size. This study presents an alternative method of measuring the size distribution of particles with aggregation behavior, particularly, by using non-invasive microscopy and image processing and analysis.

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Analysis of aggregate size as a process variable affecting ...

2011-10-1  Plant cell aggregates have long been implicated in affecting cellular metabolism in suspension culture, yet the rigorous characterization of aggregate size as a process variable and its effect on bioprocess performance has not been demonstrated. Aggregate fractionation and analysis of biomass‐associated product is commonly used to assess the effect of aggregation, but we establish

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Characterization of aggregate size in Taxus suspension ...

Plant cells grow as aggregates in suspension culture, but little is known about the dynamics of aggregation, and no routine methodology exists to measure aggregate size. In this study, we evaluate several different methods to characterize aggregate size in Taxus suspension cultures, in which aggregate diameters range from 50 to 2,000 µm, including filtration and image analysis, and develop a...

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4 Process Involved in Scale up Criteria Microbial ...

2021-8-16  E. Similar drop size distribution (d s = constant) ... In batch processing this bioreactor volume ranges between 500-5000 I capacities, operating over 1-3 weeks. ... The process parameters for scale up of plant cell cultures as listed in Table 8.5 demand low shear bioreactor for plant cell aggregates. As reported in literature plant cell ...

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Distribution of particle aggregates in the internodal ...

Distribution of particle aggregates in the internodal axolemma and adaxonal Schwann cell membrane of rodent peripheral nerve. Stolinski C, Breathnach AS, Thomas PK, Gabriel G, King RH. Freeze-fracture studies on myelinated fibres from the internodal regions of rat and mouse sciatic nerve show symmetrical particle aggregates within the adaxonal ...

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The cell size distribution of tomato fruit can be changed ...

The role of the cell cycle therein is poorly understood. Plant cyclin-dependent kinases (CDKs) are homologues of yeast cdc2, an important cell cycle regulator conserved throughout all eukaryotes. CDKA1 is constitutively expressed during the cell cycle and has dual functions in S- and M-phase progression.

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